Bioinformatics for a high-throughput deep mutational scanning protein-protein interaction assay: turning a prototype into a precision medicine toolbox Ghent University
This proposal contributes to the development of a high-throughput method to characterize the impact of nearly every mutation on specific protein-protein interactions. After bioinformatics-driven experimental optimization, this method will be applied to tumor-driver protein interactions (TP53, PTEN, PIK3R1) and results compared to large-scale tumor mutation and gene expression data. This will allow to demonstrate the major relevance for ...