A bioinformatic toolbox for the analysis and optimization of a high-throughput deep mutational scanning assay: development and case-study Ghent University
Deep Mutational Scanning (DMS) is a recent methodology for characterizing and functionally screening protein mutations by coupling a property of interest to a DNA-sequencing read-out. Via next-generation sequencing, its throughput is unmatched by traditional assays such as two-hybrid screening, which can only be realistically performed on a few variants at a time. To tackle the shortcomings of current DMS-implementations, we developed a new ...