Publications
Bedaquiline susceptibility testing of Mycobacterium tuberculosis in an automated liquid culture system Institute of Tropical Medicine
OBJECTIVES: The objective of this study was to evaluate the performance of the BACTEC MGIT960 system to test the susceptibility to bedaquiline for Mycobacterium tuberculosis complex.
METHODS: We determined the quality control (QC) range of bedaquiline using the M. tuberculosis H37Rv reference strain and the epidemiological cut-off (ECOFF) in MGIT960 and on Middlebrook 7H11 agar (M7H11) using 47 strains from bedaquiline treatment-naive ...
Bedaquiline susceptibility testing of Mycobacterium tuberculosis in an automated liquid culture system University of Antwerp
Site-directed mutagenesis of Mycobacterium tuberculosis and functional validation to investigate potential bedaquiline resistance-causing mutations University of Antwerp
Acquired bedaquiline resistance during the treatment of drug-resistant tuberculosis: a systematic review Institute of Tropical Medicine
BACKGROUND: Drug-resistant tuberculosis (DR-TB) is considered to be a public health threat and is difficult to cure, requiring a lengthy treatment with potent, potentially toxic drugs. The novel antimicrobial agent bedaquiline has shown promising results for patients with DR-TB, improving the rate of culture conversion and reducing TB-related mortality. However, increasing numbers of cases with acquired bedaquiline resistance (ABR) have been ...
In vitro evaluation of poly(lactide-co-glycolide) in situ forming gels for bedaquiline fumarate salt and pharmacokinetics following subcutaneous injection in rats University of Antwerp
Characterization of genomic variants associated with resistance to Bedaquiline and Delamanid in naive Mycobacterium tuberculosis clinical strains University of Antwerp Institute of Tropical Medicine
Bedaquiline resistance probability to guide treatment decision making for rifampicin-resistant tuberculosis University of Antwerp
MDR M. tuberculosis outbreak clone in Eswatini missed by Xpert has elevated bedaquiline resistance dated to the pre-treatment era Institute of Tropical Medicine
Background Multidrug-resistant (MDR) Mycobacterium tuberculosis complex strains not detected by commercial molecular drug susceptibility testing (mDST) assays due to the RpoB I491F resistance mutation are threatening the control of MDR tuberculosis (MDR-TB) in Eswatini. Methods We investigate the evolution and spread of MDR strains in Eswatini with a focus on bedaquiline (BDQ) and clofazimine (CFZ) resistance using whole-genome sequencing in ...
Acquired resistance of Mycobacterium tuberculosis to bedaquiline Institute of Tropical Medicine
Bedaquiline (BDQ), an ATP synthase inhibitor, is the first drug to be approved for treatment of multi-drug resistant tuberculosis in decades. In vitro resistance to BDQ was previously shown to be due to target-based mutations. Here we report that non-target based resistance to BDQ, and cross-resistance to clofazimine (CFZ), is due to mutations in Rv0678, a transcriptional repressor of the genes encoding the MmpS5-MmpL5 efflux pump. ...