Projects
Development of native comprehensive three-dimensional liquid chromatography-mass spectrometry for unravelling biomolecular interaction networks in support of post-genomic biotechnology in healthcare: 3DbioNET Vrije Universiteit Brussel
Harvesting the potential of fluorine labels and NMR spectroscopy to study the conformation of polyproline sequences and their interaction with biomolecular targets Ghent University
Proline-rich or polyproline regions play an important role in biology, often involved in proteinprotein interactions that mediate cell signaling or gene regulation. Typically present in disordered protein domains, their characterization by NMR spectroscopy is often hampered by the lack of an amide proton in Pro residues and the low resolution between resonances of the different residues. The otherwise so powerful ability of NMR to probe ...
Studying biomolecular corona-dependency of (sub)cellular interactions using patient-derived tumor models to guide ev-based therapeutic development. Ghent University
Extracellular vesicles (EV) are complex nanoparticles required for the intercellular transfer of diverse biological cargoes. Exploitation of EV as advanced drug delivery systems has sparked general interest both in academia and industry for the treatment of a multitude of diseases including cancer. However, the clinical application of EV-based therapeutics is hampered by the lack of knowledge on their (sub)cellular interactions and the ...
High throughput analysis of biomolecular interactions KU Leuven
In this grant we apply for instruments that allow us to measure interactions between biological macromolecules, like proteins, DNA and lipids. These transient interactions between molecules are thought to be the basis of all cellular processes and thus life itself. The combination of instruments requested here was selected because: (A) It allows to measure not only the strength of the interactions but also the speed at which molecules get ...
Visualization of biomolecular interactions and sensing in living systems with super-resolution fluorescence microscopy. KU Leuven
Fluorescence microscopy is a powerful method to study living systems with high spatial and temporal resolution. However, the resolution of a conventional microscope is limited by diffraction, which precludes the direct visualization of many biological processes occurring at their small scale. During the last decade, several super-resolution fluorescence microscopy methods have been developed that break this diffraction limit and offer a new ...
Super resolution fluorescence microscopy of biomolecular interactions with photochromic biosensors and complementation. KU Leuven
Fluorescence microscopy techniques are indispensable research tools that have enabled live cell imaging with low invasiveness, high sensitivity, and high spatiotemporal resolution. As a result, fluorescence microscopy has revolutionized our understanding of the inner workings of single cells and whole organisms. An integral part of the imaging performance is the fluorescent labels, as the quality of the labels influences the quality of the ...