Generation of autologous T cells against acute myeloid leukemia by transduction of hematopoietic stem cells with T cell receptor genes and subsequent differentiation to T cells Ghent University
Hematopoietic stem cells will be transduced to express a T Cell Receptor. This receptor will be optained by immunizing against WT1 tumor antigen. The T cell receptor genes will be cloned from WT1 speciific T cells and subsequently used for transduction of the stem cells. Stem cells will be differentiated in vitro to T cells and functionality will be assayed in an in vivo animal model
The hypoxia responsive protein BNIP3 plays an important role in promoting cell death and/or autophagy, ultimately resulting in a cancer type-dependent, tumour-enhancer or tumour-suppressor activity. We previously reported that in melanoma cells, BNIP3 regulates cellular morphology, mitochondrial clearance, cellular viability and maintains protein expression of CD47, a pro-cancerous, immunosuppressive 'don't eat me' signal. Surface-exposed ...
Recognition of alfaherpesvirus envelope glycoproteins by dendritic cells and natural killer cells Ghent University
Dendritic cells and natural killer cells are crucial for an efficient immune response against alphaherpesviruses. These cells are able, via an unknown mechanism, to recognize alphaherpesvirus envelope glycoproteins. The aim of the project proposal is to unravel this mechanism, which will lead to new virological and immunological insights that may lead to new strategies to interfere with alphaherpesvirus infections.
Thymic reprogramming: Systemic inflammation changes the cellular output of the thymus from T cells to NK cells and iron-binding myeloid cells Ghent University
IL-12 and IL-18 are synergistically acting cytokines that are involved in the pathology of both viral infection and sepsis. By culturing ex vivo thymic lobes from neonatal mice, I was able to investigate the cells exiting the thymus following IL-12 and IL-18 stimulation. Surprisingly, this cytokine combination led to the exit of a massive amount of NK cells and myeloid cells into the supernatant of the organ culture. We have been able to ...
Study of human lymphoid cell differentiation using gene-modified embryonic stem cells and induced pluripotent stem cells from immunodeficiency patients Ghent University
Pluripotent stem cells will be generated from patients with an inborn immune (T/NK) deficiency. In addition, gene modified human embryonic stem cells will be generated for lineage tracing and deletion mutants. Using these cell lines T and NK differentiation will be studied and the effect of the targeted genes on differentiation will be analyzed.