GENERATION OF ARTIFICIAL NUCLEIC ACID SYSTEMS FOR BIOTECHNOLOGY AND THERAPEUTICS KU Leuven
1、Design and preparation of artificial DNA and RNA molecules. Search for the optimal structure. Synthesis, purification and characterization of sequences. Optimization of PCR and reverse transcription reaction conditions. 2、Enzymatic methods screening for XNA assembly. Selection of the optimal enzymes (XNA-dependent nucleases, ligases, and polymerases) for cloning and assembly of genomic XNA fragments and vectors. Analysis of the product. ...