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Unraveling the effect of the aptamer complementary element on the performance of duplexed aptamers: a thermodynamic study KU Leuven University of Antwerp
Duplexed aptamers (DAs) are widespread aptasensor formats that simultaneously recognize and signal the concentration of target molecules. They are composed of an aptamer and aptamer complementary element (ACE) which consists of a short oligonucleotide that partially inhibits the aptamer sequence. Although the design principles to engineer DAs are straightforward, the tailored development of DAs for a particular target is currently based on trial ...
Chemical modification of aptamers for increased binding affinity in diagnostic applications : current status and future prospects Ghent University
Aptamers targeting different functional groups of 17 beta-estradiol Hasselt University KU Leuven
Aptamers, short synthetic ssDNA or RNA molecules with a specific three-dimensional structure, are promising recognition elements in biosensor technology. In vitro generation of aptamers with high sensitivity and specificity toward a broad range of analytes has been achieved using the systematic evolution of ligands by exponential enrichment (SELEX) process. This iterative pathway of aptamer generation consists of sequential positive and ...
On-line aptamer affinity solid-phase extraction capillary electrophoresis-mass spectrometry for the analysis of blood alpha-synuclein Ghent University
Tethered imidazole mediated duplex stabilization and its potential for aptamer stabilization Ghent University
In vitro selection and characterization of DNA aptamers recognizing chloramphenicol Research Institute for Agriculture, Fisheries and Food University of Antwerp
Chloramphenicol (Cam), although an effective antibiotic, has lost favour due to some fatal side effects. Thus there is an urgent need for rapid and sensitive methods to detect residues in food, feed and environment. We engineered DNA aptamers that recognize Cam as their target, by conducting in vitro selections. Aptamers are nucleic acid recognition elements that are highly specific and sensitive towards their targets and can be synthetically ...
Selection and characterization of PCB-binding DNA aptamers Research Institute for Agriculture, Fisheries and Food University of Antwerp
Polychlorinated biphenyls (PCBs) are persistent organic pollutants (POPs) that resist natural degradation and bioaccumulate in nature. Combined with their toxicity, this leads them to cause cancer and other health hazards. Thus, there is a vital need for rapid and sensitive methods to detect PCB residues in food and in the environment. In this study, PCB-binding DNA aptamers were developed using PCB72 and PCB106 as targets for aptamer selection. ...
Characterization of an HNA aptamer suggests a non-canonical G-quadruplex motif KU Leuven
Nucleic acids not only form the basis of heredity, but are increasingly a source of novel nano-structures, -devices and drugs. This has spurred the development of chemically modified alternatives (xeno nucleic acids (XNAs)) comprising chemical configurations not found in nature to extend their chemical and functional scope. XNAs can be evolved into ligands (XNA aptamers) that bind their targets with high affinity and specificity. However, ...
Development of a suitable bioreceptor for C-reactive protein: Aptamer selection, validation & sensor development Hasselt University
Not many research areas are as evolving as biosensor research. Started in the fifties, it is now expanded to a technology-driven, multidisciplinary field with large impact on society and hence commercial interest. A myriad of applications exists for a device that very specifically and sensitively measures the presence and concentration of a target molecule of choice, e.g. in environmental monitoring (contaminants, pesticides), food analysis ...