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Chromosomal aneuploidy in embryos conceived with unstimulated cycle IVF. Vrije Universiteit Brussel

Willem Verpoest, Bart Fauser, Evangelos Papanikolaou, Catherine Staessen, Lisbet Van Landuyt, Herman Tournaye, Ingeborg Liebaers, Paul Devroey
There is an ever increasing trend in reproductive medicine to reduce the intensity of ovarian stimulation for in vitro fertilization (IVF) and to restrict the number of embryos that are transferred into the uterine cavity. Recent findings suggest that the magnitude of ovarian stimulation affects the proportion of euploid embryos. As a result of the restriction in the number of embryos transferred, it becomes even more important to select the ...

Four layer multi-omics reveals molecular responses to aneuploidy in Leishmania Hasselt University Institute of Tropical Medicine

Bart Cuypers, Pieter Meysman, Ionas Erb, Wout Bittremieux, Dirk VALKENBORG, Geert Baggerman, Inge Mertens, Shyam Sundar, Basudha Khanal, Cedric Notredame, et al.
Aneuploidy causes system-wide disruptions in the stochiometric balances of transcripts, proteins, and metabolites, often resulting in detrimental effects for the organism. The protozoan parasite Leishmania has an unusually high tolerance for aneuploidy, but the molecular and functional consequences for the pathogen remain poorly understood. Here, we addressed this question in vitro and present the first integrated analysis of the genome, ...

Chemically induced aneuploidy in germ cells. Part II of the report of the 2017 IWGT workgroup on assessing the risk of aneugens for carcinogenesis and hereditary diseases Vrije Universiteit Brussel

Francesca Pacchierotti, Kenichi Masumura, David A Eastmond, Azeddine Elhajouji, Roland Froetschl, Micheline Volders, Anthony Lynch, Maik Schuler, David Tweats, Francesco Marchetti, et al.

As part of the 7th International Workshops on Genotoxicity Testing held in Tokyo, Japan in November 2017, a workgroup of experts reviewed and assessed the risk of aneugens for human health. The present manuscript is one of three manuscripts from the workgroup and reports on the unanimous consensus reached on the evidence for aneugens affecting germ cells, their mechanisms of action and role in hereditary diseases. There are 24 chemicals with ...

Preimplantation genetic testing for aneuploidy by microarray analysis of polar bodies in advanced maternal age Vrije Universiteit Brussel

Willem Verpoest, Catherine Staessen, Patrick M Bossuyt, Veerle Goossens, Gheona Altarescu, Maryse Bonduelle, Martha Devesa, Talia Eldar-Geva, Luca Gianaroli, Georg Griesinger, et al.

STUDY QUESTION: Does preimplantation genetic testing for aneuploidy (PGT-A) by comprehensive chromosome screening (CCS) of the first and second polar body to select embryos for transfer increase the likelihood of a live birth within 1 year in advanced maternal age women aged 36-40 years planning an ICSI cycle, compared to ICSI without chromosome analysis?

SUMMARY ANSWER: PGT-A by CCS in the first and second polar body to select euploid ...

Analysis of parental contribution for aneuploidy detection (APCAD) Vrije Universiteit Brussel

Pieter Verdyck, Veerle Berckmoes, Sven Van Laere, K. Keymolen, Catharina Olsen, Martine De Rijcke

RESEARCH QUESTION: Can (mosaic) aneuploidy be reliably detected in preimplantation embryos after multiple displacement amplification and single nucleotide polymorphism detection, independent of haplotyping and copy number detection, with a new method 'analysis of parental contribution for aneuploidy detection' or 'APCAD'?

DESIGN: This method is based on the maternal contribution, a parameter that reflects the proportion of DNA that is ...

Higher limb asymmetry in deceased human fetuses and infants with aneuploidy University of Antwerp

Jessica Bots, Claartje ten Broek, Jeroen A.M. Belien, Marianna Bugiani, Frietson Galis, Stefan Van Dongen
Aneuploidies cause gene-dosage imbalances that presumably result in a generalized decreased developmental homeostasis, which is expected to be detectable through an increase in fluctuating asymmetry (FA) of bilateral symmetric traits. However, support for the link between aneuploidy and FA is currently limited and no comparisons among different aneuploidies have been made. Here, we study FA in deceased human fetuses and infants from a 20-year ...

Evolution of aneuploidy up to Day 4 of human preimplantation development Vrije Universiteit Brussel

Afroditi Mertzanidou, Claudia Spits, Ha Nguyen Thi, Hilde Van De Velde, Karen Sermon
Study question: What is the incidence of aneuploidy and mosaicism in all cells of top-quality Day-4 embryos analysed by array-based comparative genomic hybridization (array CGH)?
Summary answer: Our data show extensive abnormalities in Day-4 embryos.
What is known already: Numerous studies on human embryos at Day 3 and Day 5 of development show that they frequently contain aneuploid cells and are mosaic, although Day-5 embryos ...

The TLX1 oncogene drives aneuploidy in T cell transformation KU Leuven Ghent University

Kim De Keersmaecker
The TLX1 oncogene (encoding the transcription factor T cell leukemia homeobox protein-1) has a major role in the pathogenesis of T cell acute lymphoblastic leukemia (T-ALL). However, the specific mechanisms of T cell transformation downstream of TLX1 remain to be elucidated. Here we show that transgenic expression of human TLX1 in mice induces T-ALL with frequent deletions and mutations in Bcl11b (encoding B cell leukemia/lymphoma-11B) and ...

Aging rather than aneuploidy affects monoamine neurotransmitters in brain regions of Down syndrome mouse models University of Antwerp

Alain D. Dekker, Yannick Vermeiren, Christelle Albac, Eva Lana-Elola, Sheona Watson-Scales, Dorota Gibbins, Tony Aerts, Debby Van Dam, Elizabeth M.C. Fisher, Victor L.J. Tybulewicz, et al.
Altered concentrations of monoamine neurotransmitters and metabolites have been repeatedly found in people with Down syndrome (DS, trisomy 21). Because of the limited availability of human post-mortem tissue, DS mouse models are of great interest to study these changes and the underlying neurobiological mechanisms. Although previous studies have shown the potential of Ts65Dn mice the most widely used mouse model of DS to model noradrenergic ...

Flow cytometry peripheral blood micronucleus test in vivo: Determination of potential thresholds for aneuploidy induced by spindle poisons Vrije Universiteit Brussel

Zoryana Cammerer, Martin M Schumacher, Micheline Volders, Willi Suter, Azddine Elhajouji
Non-DNA binding genotoxins (e.g., aneugens), unlike DNA-binding genotoxins, are theoretically expected to show thresholded concentration-effect response curves. This is a major issue in genetic toxicology testing because the identification of thresholds in vivo can provide a safety margin for exposure to a particular compound. In the current study we measured micronucleus induction by flow cytometry to determine the dose-response curves for ...