Publications
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A protocol for a turbidimetric assay using a Saccharomyces cerevisiae thiamin biosynthesis mutant to estimate total vitamin B1 content in plant tissue samples Ghent University
Background: Understanding thiamin (thiamine; vitamin B1) metabolism in plants is crucial, as it impacts plant nutritional value as well as stress tolerance. Studies aimed at elucidating novel aspects of thiamin in plants rely on adequate assessment of thiamin content. Mass spectrometry-based methods provide reliable quantification of thiamin as well as closely related biomolecules. However, these techniques require expensive equipment and ...
Harmonization and qualification of an IFN-γ Enzyme-Linked ImmunoSpot assay (ELISPOT) to measure influenza-specific cell-mediated immunity within the FLUCOP consortium Ghent University
Influenza continues to be the most important cause of viral respiratory disease, despite the availability of vaccines. Today's evaluation of influenza vaccines mainly focuses on the quantitative and functional analyses of antibodies to the surface proteins haemagglutinin (HA) and neuraminidase (NA). However, there is an increasing interest in measuring cellular immune responses targeting not only mutation-prone surface HA and NA but also ...
Diagnostic performance of the loop-mediated isothermal amplification (LAMP) based illumigene (R) malaria assay in a non-endemic region Ghent University Institute of Tropical Medicine KU Leuven
Optimization of the oligonucleotide ligation assay, a rapid and inexpensive test for detection of HIV-1 drug resistance mutations, for non-north American variants Institute of Tropical Medicine
Performance of a commercial Swine influenza virus H1N1 and H3N2 antibody enzyme-linked immunosorbent assay in pigs experimentally infected with European influenza viruses Ghent University
The IDEXX Swine influenza virus H1N1 and H3N2 enzyme-linked immunosorbent assays (ELISAs) are used worldwide, but their capacity to detect antibodies to European Swine influenza viruses (SIVs) has not been documented. A total or 313 well-defined sera from SIV seronegative pigs and pigs experimentally infected with European SIVs were used to compare the performance of both ELISAs and the hemagglutination inhibition (HI) test. The ELISAs largely ...
The cytokinesis-block micronucleus assay on human isolated fresh and cryopreserved peripheral blood mononuclear cells Ghent University
Assaying Cellular Viability Using the Neutral Red Uptake Assay Vrije Universiteit Brussel
The neutral red uptake assay is a cell viability assay that allows in vitro quantification of xenobiotic-induced cytotoxicity. The assay relies on the ability of living cells to incorporate and bind neutral red, a weak cationic dye, in lysosomes. As such, cytotoxicity is expressed as a concentration-dependent reduction of the uptake of neutral red after exposure to the xenobiotic under investigation. The neutral red uptake assay is mainly used ...
An aphidicolin-block nucleotide excision repair assay measuring DNA incision and repair capacity Vrije Universiteit Brussel
The objective of the present study was to develop a cellular phenotype assay for nucleotide excision repair (NER), using benzo[a]pyrene diol epoxide (BPDE) as model mutagen. Since in vitro exposure to BPDE may lead to DNA strand breaks resulting from both direct interaction with DNA and incisions introduced by the repair enzymes, we aimed to discriminate between both types of breaks using the comet assay and quantified the DNA strand breaks ...