Furan and porphyrin containing crosslinking ligands for covalent stabilisation of G quadruplexes as cell-permeable anti-cancer aptamers Ghent University
Using a variety established DNA crosslinking nucleobase-analogues a method will be developed for the stapling of the 5’ and 3’ ends of G-quadruplex forming aptamer sequences. This staple will in theory convey several benefits such as conformational locking and improved formation kinetics. Crosslinked aptamers will then be assessed for improvements in these properties compared to wild type oligonucleotides.