Detection of distinct molecules using fiber optic sensor KU Leuven
The invention relates to a method (10) for detecting distinct target molecules (21) in a sample (26). The method comprises providing (11) a fiber optic sensor (23) functionalized with distinct affinity probes (24) immobilized on a detection surface (25), each probe specifically binding to a corresponding molecule (21) to be detected. The method further comprises providing (12) reagent fluids (26), each comprising an affinity reagent(s) (27) for binding simultaneously with a corresponding probe (24) to the corresponding molecule (21). Each reagent is bound to a metal nanoparticle (28). Each ...