Publications
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2D versus 3D cross-correlation-based radial and circumferential strain imaging in a 3D atherosclerotic carotid artery model using ultrafast plane wave ultrasound Ghent University
Three-dimensional vascular strain estimation is crucial for assessment of the location of high strain regions in the carotid artery (CA) and the identification of vulnerable plaque features. This study compares 2D vs. 3D displacement estimation in terms of radial and circumferential strain using simulated ultrasound images of a 3D atherosclerotic CA model at the bifurcation embedded in surrounding tissue. The 3D finite element model (FEM) of a ...
Non-Rigid Image Registration for the Assessment of Myocardial Deformation from 3D Echocardiography (Niet-rigide beeldregistratie voor het bepalen van de hartspiervervorming met behulp van 3D ultrasone beelden) KU Leuven
Cardiovascular diseases are currently the major cause of death in the wo rld. Cardiac imaging therefore plays an essential role in the diagnosis, management and follow-up of patients with any suspected or known cardio vascular disease. Ultrasound (US) imaging is a well established imaging modality in daily clinical practice for the evaluation of cardiac morpho logy and function by measuring cardiac wall motion and deformation (i.e. strain). ...
Generating human skeletal myoblast spheroids for vascular myogenic tissue engineering Hasselt University
Engineered myogenic microtissues derived from human skeletal myoblasts offer unique opportunities for varying skeletal muscle tissue engineering applications, such as in vitro drug-testing and disease modelling. However, more complex models require the incorporation of vascular structures, which remains to be challenging. In this study, myogenic spheroids were generated using a high-throughput, non-adhesive micropatterned surface. Since ...
Cardiac shear wave speed estimation in 3D : an in silico and in vivo study Ghent University
Cardiac shear wave elastography (SWE) is a promising technique that can give additional information about tissue mechanical properties and, potentially, improve diagnosis of cardiac disease. The 'passive' approach to SWE tracks shear waves (SWs) after valve closure typically in a parasternal long axis view using 2D high frame rate (HFR) imaging. However, 2D methods provide an incomplete picture of the localization of the wave excitation source ...
Polymeric reinforcements for cellularized collagen-based vascular wall models : influence of the scaffold architecture on the mechanical and biological properties Ghent University
A previously developed cellularized collagen-based vascular wall model showed promising results in mimicking the biological properties of a native vessel but lacked appropriate mechanical properties. In this work, we aim to improve this collagen-based model by reinforcing it using a tubular polymeric (reinforcement) scaffold. The polymeric reinforcements were fabricated exploiting commercial poly (epsilon-caprolactone) (PCL), a polymer already ...
Dental tissue regeneration in children, Can we mimic nature? KU Leuven
The general aim of this PhD project was to bridge part of the gap between in vitro studies and the clinical application for dental tissue regeneration with the main use of a cell-free approach. This aim was approached systematically, on the one hand, through the study of two clinical models involving tissue repair and regeneration namely tooth autotransplantation (TAT) and regenerative endodontic treatment (RET). On the other hand, by exploring ...
Synchrotron-based phase contrast imaging of cardiovascular tissue in mice-grating interferometry or phase propagation? Ghent University
Synchrotron-based x-ray phase-contrast imaging allows for detailed 3D insight into the microstructure of soft tissue and is increasingly used to improve our understanding of mouse models of cardiovascular disease. Two techniques dominate the field: grating interferometry, with superior density contrast at mid to lower microscopic resolutions, and propagation-based phase contrast, facilitating high-resolution tissue imaging. The choice between ...
Mouse in vitro spermatogenesis on alginate-based 3D bioprinted scaffolds Vrije Universiteit Brussel
In vitro spermatogenesis (IVS) has already been successfully achieved in rodents by organotypic and soft matrix culture systems. However, the former does not allow single cell input, and the latter presents as a simple thick layer in which all cells are embedded. We explored a new culture system using a mouse model by employing an alginate-based hydrogel and 3D bioprinting, to control scaffold design and cell deposition. We produced ...
Automatic analysis of the 3-D microstructure of fruit parenchyma tissue using X-ray micro-CT explains differences in aeration KU Leuven
Background: 3D high-resolution X-ray imaging methods have emerged over the last years for visualising the anatomy of tissue samples without substantial sample preparation. Quantitative analysis of cells and intercellular spaces in these images has, however, been difficult and was largely based on manual image processing. We present here an automated procedure for processing high-resolution X-ray images of parenchyma tissues of apple (Malus × ...