Publications
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Imaging of molecular oxygen at subcellular resolution in mammalian cell culture : from 2D to 3D models Ghent University
Organotypic 3D cell culture models : using the rotating wall vessel to study host–pathogen interactions Ghent University
Towards Self-Regulated Bioprocessing: A Compact Benchtop Bioreactor System for Monitored and Controlled 3D Cell and Tissue Culture KU Leuven
Bioreactors are crucial tools for the manufacturing of living cell-based tissue engineered products. However, to reach the market successfully, higher degrees of automation, as well as a decreased footprint still need to be reached. In this study, the use of a benchtop bioreactor for in vitro perfusion culture of scaffold-based tissue engineering constructs is assessed. A low-footprint benchtop bioreactor system is designed, comprising a ...
Fast compressive lens-free tomography for 3D biological cell culture imaging KU Leuven
We present a compressive lens-free technique that performs tomographic imaging across a cubic millimeter-scale volume from highly sparse data. Compared with existing lens-free 3D microscopy systems, our method requires an order of magnitude fewer multi-angle illuminations for tomographic reconstruction, leading to a compact, cost-effective and scanningfree setup with a reduced data acquisition time to enable high-throughput 3D imaging of dynamic ...
CRISPR/Cas9-edited ROS1 + non-small cell lung cancer cell lines highlight differential drug sensitivity in 2D vs 3D cultures while reflecting established resistance profiles University of Antwerp
Introduction The study of resistance-causing mutations in oncogene-driven tumors is fundamental to guide clinical decisions. Several point mutations affecting the ROS1 kinase domain have been identified in the clinical setting, but their impact requires further exploration, particularly in improved pre-clinical models. Given the scarcity of solid pre-clinical models to approach rare cancer subtypes like ROS1 + NSCLC, CRISPR/Cas9 technology ...
Optical methods for biophysical stimulation and monitoring in 2D and 3D cell cultures KU Leuven
During my PhD session, I will focus on exploring the cellular responses to localized temperature and electromagnetic stimulation. In order to realize this, first of all, light-addressable neuroactive matrices will be constructed by combining Digital micro-mirror device (DMD) with microscope in order to realize dynamic stimulation in neuronal matrices. For conventional microscope, confined with its illumination pattern, it's hard to realize ...
Interactions of Pluronic nanocarriers with 2D and 3D cell cultures: effects of PEO block length and aggregation state Ghent University
Role of preconditioning with oxygen and glucose deprivation in promoting differentiation of dental pulp stem cells in 3D culture Ghent University
Role of preconditioning with oxygen and glucose deprivation in promoting differentiation of dental pulp stem cells in 3D culture Irina A. Okkelman, Winter Vandenberghe, Ruslan I. Dmitriev Tissue Engineering and Biomaterials Group, Department of Human Structure and Repair, Faculty of Medical and Health Sciences, Ghent University, Ghent 9000, Belgium Dental pulp stem cells (DPSC) are promising for bone tissue engineering and repair. To support ...
Towards Mimicking the Foetal Liver Niche: Engineering 3D Hydrogel Systems for in-vitro Culture of Hematopoietic Stem Cells KU Leuven
Within my PhD, I will be working on the development of 3D culture systems for the in-vitro culture of hematopoietic stem/progenitor cells (HSPCs), which are a small population of stem cells giving rise to all blood cells during the lifespan of the organism. These 3D culture system will be designed to mimics key biophysical and biological features of the fetal liver (FL) niche, the home place of these stem cells during mammalian development. In ...