Projects
Development of isogenic human embryonic stem cell-derived 3D neuro-immune cell culture models: pre-clinical evaluation of microglia and macrophage immunomodulation for stroke treatment. University of Antwerp
3D multicellular culture systems for real time insights in physical and chemical signals underlying stem cell morphogenesis and lineage fateAcronym: 3DMuSyC KU Leuven
Generation of iPSC-derived 3D neuro-immune cell culture models for study and modulation of neuro-inflammatory processes. University of Antwerp
The IncuCyte S3/Organoid platform for live cell culture imaging and kinetic multiparameter analysis of state-of-the-art 3D organoid models and complex tissue mimics KU Leuven
Optical methods for biophysical stimulation and monitoring in 2D and 3D cell cultures KU Leuven
During my PhD session, I will focus on exploring the cellular responses to localized temperature and electromagnetic stimulation. In order to realize this, first of all, light-addressable neuroactive matrices will be constructed by combining Digital micro-mirror device (DMD) with microscope in order to realize dynamic stimulation in neuronal matrices. For conventional microscope, confined with its illumination pattern, it’s hard to realize ...
Towards Mimicking the Foetal Liver Niche: Engineering 3D Hydrogel Systems for in-vitro Culture of Hematopoietic Stem Cells KU Leuven
Within my PhD, I will be working on the development of 3D culture systems for the in-vitro culture of hematopoietic stem/progenitor cells (HSPCs), which are a small population of stem cells giving rise to all blood cells during the lifespan of the organism. These 3D culture system will be designed to mimics key biophysical and biological features of the fetal liver (FL) niche, the home place of these stem cells during mammalian development. ...
Recellularization of decellularized rad liver with human hepatic cells as a novel 3D culture model for pharmaco-toxicological research Vrije Universiteit Brussel
In vitro functional formation of liver sinusoidal endothelial cells in 3D culture system. KU Leuven
Liver sinusoidal endothelial cells (LSECs) are highly specialized endothelial cells that form the barrier between the blood and hepatocytes and hepatic stellate cells (HSCs). LSECs lack a basement membrane and are fenestrated, allowing macromolecule transport across this barrier. In liver inflammation, LSECs dedifferentiate (also called sinusoidal capillarization), form a basement membrane and lose fenestrae, which further promotes fibrosis. ...