Confocal and two-photon Microscope (Nikon A1R-MP) Ghent University
Confocal laser scanning microscopy (CLSM) is a widely used technique in the life sciences for making high-quality images of biological samples. The samples are illuminated by a focused laser beam which is scanned over the sample. in order to visualize fluorescent markers. Emission from each position is detected by a point detector and a pinhole removes out of focus emission light to improve contrast and resolution. CLSM results in optical sectioning and a 3D reconstruction of the sample is made.
Two-photon microscopy is available on this instrument making use of a MaiTai Deep ...