NIR-Sensitivity-Enhanced Gated Image Sensor for Fluorescence Lifetime Imaging Microscopy (FLIM) KU Leuven
Because of the deep tissue penetration and the lower influence from the autofluorescence background, fluorescence lifetime imaging microscopy (FLIM) in the near-infrared (NIR) range becomes attractive for bioimaging. However, to reconstruct the fluorescence decay profile with the time-resolved technique, repetitive short excitation pulses are required, and a large number of light exposures easily result in the photobleaching of the ...