Evaluation of different strategies to produce Vibrio cholerae ParE2 toxin Vrije Universiteit Brussel Flanders Institute for Biotechnology
Toxin-antitoxin (TA) systems are small operons that are omnipresent in bacteria and archaea with suggested roles in stabilization of mobile genetic elements, bacteriophage protection, stress response and possibly persister formation. A major bottleneck in the study of TA toxins is the production of sufficient amounts of well-folded, functional protein. Here we examinate alternative approaches for obtaining the VcParE2 toxin from Vibrio cholerae. ...