Publications
Patterned dextran ester films as a tailorable cell culture platform KU Leuven
The elucidation of cell-surface interactions and the development of model platforms to help uncover their underlying mechanisms remains vital to the design of effective biomaterials. To this end, dextran palmitates with varying degrees of substitution were synthesised with a multipurpose functionality: an ability to modulate surface energy through surface chemistry, and an ideal thermal behaviour for patterning. Herein, dextran palmitate films ...
Robust gene expression programs underlie recurrent cell states and phenotype switching in melanoma KU Leuven
Melanoma cells can switch between a melanocytic and a mesenchymal-like state. Scattered evidence indicates that additional intermediate state(s) may exist. Here, to search for such states and decipher their underlying gene regulatory network (GRN), we studied 10 melanoma cultures using single-cell RNA sequencing (RNA-seq) as well as 26 additional cultures using bulk RNA-seq. Although each culture exhibited a unique transcriptome, we identified ...
Integration of Interdigitated Electrodes in Split-Ring Resonator for Detecting Liquid Mixtures KU Leuven
Novel implantable pressure and acceleration sensor for bladder monitoring KU Leuven
OBJECTIVES: To test the hypothesis that an implantable sensing system containing accelerometers can detect small-scale autonomous movements, also termed micromotions, which might be relevant to bladder physiology. METHODS: We developed a 6-mm submucosal implant containing a pressure sensor (MS5637) and a triaxial accelerometer (BMA280). Sensor prototypes were tested by implantation in the bladders of Gottingen minipigs. Repeated awake voiding ...
Bendable Piezoelectric Micromachined Ultrasound Transducer (PMUT) Arrays Based on Silicon-On-Insulator (SOI) Technology KU Leuven
This paper presents the design and fabrication method to render a silicon-on-insulator (SOI)-based pMUT array bendable. The proposed method can be applied to silicon-based pMUT arrays with different acoustical specifications. A bendable array based on the proposed method is highly conformal to the target structure. The bendable structure is made out of several silicon islands containing a pMUT array and are connected to each other by silicon ...
Novel Fabrication Process for Integration of Microwave Sensors in Microfluidic Channels KU Leuven
This paper presents a novel fabrication process that allows integration of polydimethylsiloxane (PDMS)-based microfluidic channels and metal electrodes on a wafer with a micrometer-range alignment accuracy. This high level of alignment accuracy enables integration of microwave and microfluidic technologies, and furthermore accurate microwave dielectric characterization of biological liquids and chemical compounds on a nanoliter scale. The ...
Digital Microfluidics for Single Bacteria Capture and Selective Retrieval Using Optical Tweezers KU Leuven
When screening microbial populations or consortia for interesting cells, their selective retrieval for further study can be of great interest. To this end, traditional fluorescence activated cell sorting (FACS) and optical tweezers (OT) enabled methods have typically been used. However, the former, although allowing cell sorting, fails to track dynamic cell behavior, while the latter has been limited to complex channel-based microfluidic ...
System for recording from multiple flexible polyimide neural probes in freely behaving animals KU Leuven
OBJECTIVE: Long-term electrophysiological recordings of neural activity in freely behaving animals are indispensable to advance the understanding of complex brain function. It is a technical challenge to chronically monitor the detailed activity across multiple distributed brain regions in freely behaving animals over a period of months. Here we present a new implant for inserting multiple flexible polyimide probes into freely behaving rats for ...