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Next generation point-of-care test for therapeutic drug monitoring of adalimumab in patients diagnosed with autoimmune diseases KU Leuven

Henry Ordutowski, Francesco Dal Dosso, Wout De Wispelaere, Charlotte Van Tricht, Nick Geukens, Dragana Spasic, Jeroen Lammertyn
Therapeutic drug monitoring (TDM) of adalimumab (ADM) at the point-of-care (POC) is key to prevent loss of response but has not been accomplished to date because true POC testing solutions are still lacking. Here, we present a novel “whole blood in – result out” self-powered microfluidic chip for detecting ADM within 30 min to enable TDM at POC. Hereto, we first demonstrated on-chip plasma separation from whole blood, followed by downscaling an ...

Point-of-care therapeutic drug monitoring of adalimumab by integrating a FO-SPR biosensor in a self-powered microfluidic cartridge KU Leuven

Henry Ordutowski, Charlotte Van Tricht, Mattijs Bulcaen, Marta Ciwinska, Caro Gutierrez Cisneros, Xander Janssens, Nick Geukens, Debby Thomas, Francesco Dal Dosso, Dragana Spasic, et al.
Disease treatment with advanced biological therapies such as adalimumab (ADM), although largely 16beneficial,is still costly and suffersfrom loss of response. To tackle these aspects, therapeutic drug 17monitoring (TDM) isproposed toimprove treatment dosing and efficacy, but is often associated 18with long sampling-to-result workflows. Here, we presentan in-house constructed ADM-sensor, 19allowing TDM of ADMat the doctor’s office. This biosensor ...

Engineered tracrRNA for enabling versatile CRISPR-dCas9-based biosensing concepts KU Leuven

Seppe Driesen, Karen Leirs, Jeroen Lammertyn, Dragana Spasic
In recent years, CRISPR-Cas (stands for: clustered regularly interspaced short palindromic repeats - CRISPR associated protein) based technologies have gained increasing attention in the biosensing field. Thanks to excellent sequence specificity, their use is of particular interest for detecting nucleic acid (NA) targets. In this context, signal generation and amplification can be realized by employing the cis-cleavage activity of the Cas9 ...

Influence of temperature during incubation on the mRNA levels of temperature sensitive ion channels in the brain of broiler chicken embryos. KU Leuven

Sara Verlinden, Tomas Norton, Mona Larsen, Nadia Everaert
Thermosensation is crucial for the survival of any organism. In animals, changes in brain temperature are detected via sensory neurons, their cell bodies are located in the trigeminal ganglia. Transient receptor potential (TRP) ion channels are the largest temperature sensing family. In mammals, 11 thermoTRPs are known, as in poultry, there are only three. This research further elucidates TRP mRNA expression in the brain of broiler embryo's. ...

Fast detection of SARS-CoV-2 variants including Omicron using one-step RT-PCR and Sanger sequencing. KU Leuven

Annabel Rector, Piet Maes, Bert Vanmechelen
SARS-CoV-2 has kept the world in suspense for almost 2 years now. The virus spread rapidly worldwide and several variants of concern have emerged: Alpha, Beta, Gamma, Delta and recently Omicron. A rapid method to detect key mutations is needed because these variants may jeopardize the effectiveness of immune protection following vaccination or past infection. This article describes an easy, cheap and fast method for the detection of mutations in ...

Secondary-metabolites fingerprinting of Argania spinosa kernels using liquid chromatography-mass spectrometry and chemometrics, for metabolite identification and quantification as well as for geographic classification Vrije Universiteit Brussel

Mourad Kharbach, Johan Viaene, Huiwen Yu, Rabie Kamal, Ilias Marmouzi, Abdelaziz Bouklouze, Yvan Vander Heyden

Argan (Argania spinosa L.) fruit kernels' composition has been poorly studied and received less research intensity than the resulting Argan oil. The Moroccan Argan kernels contain a wealth of metabolites and can be investigated for nutritional and health aspects as well as for economic benefits. Ultra-Performance Liquid Chromatography Mass Spectrometry (UPLC-MS) was employed to trace the geographical origin of Argan kernels based on ...

Ecology and evolution of antibiotic persistence KU Leuven

Laure Verstraete, Bram Van den Bergh, Natalie Verstraeten, Jan Michiels
Bacteria have at their disposal a battery of strategies to withstand antibiotic stress. Among these, resistance is a well-known mechanism, yet bacteria can also survive antibiotic attack by adopting a tolerant phenotype. In the case of persistence, only a small fraction within an isogenic population switches to this antibiotic-tolerant state. Persistence depends on the ecological niche and the genetic background of the strains involved. ...

Simultaneously assessing amplitude and temporal effects in biomechanical trajectories using nonlinear registration and statistical nonparametric mapping. KU Leuven

Jos Vanrenterghem
Biomechanical trajectories generally embody amplitude and temporal effects, but these effects are often analyzed separately. Here we demonstrate how amplitude-phase separation techniques from the statistics literature can be used to simultaneously analyze both. The approach hinges on nonlinear registration, which temporally warps trajectories to minimize timing effects, and the resulting optimal time warps can be combined with the resulting ...

F/YGG-motif is an intrinsically disordered nucleic-acid binding motif Vrije Universiteit Brussel

Joris Van Lindt, Tamas Lazar, Donya Pakravan, Manon Demulder, Attila Meszaros, Ludo Van Den Bosch, Dominique Maes, Peter Tompa

Heterogeneous nuclear ribonucleoproteins (hnRNP) function in RNA processing, have RNA-recognition motifs (RRMs) and intrinsically disordered, low-complexity domains (LCDs). While RRMs are drivers of RNA binding, there is only limited knowledge about the RNA interaction by the LCD of some hnRNPs. Here, we show that the LCD of hnRNPA2 interacts with RNA via an embedded Tyr/Gly-rich region which is a disordered RNA-binding motif. RNA binding is ...