Delivery of functional macromolecules, such as therapeutic nucleic acids, into cells requires overcoming the cell membrane. Photoporation is a recently developed technology that allows to transiently permeabilize the cell membrane through a combination of
Using molecular proximity to refine synapse quantification in neuronal cultures. University of Antwerp
Evaluating the potential of nanomaterials for IP administration of chemotherapeutics and nucleic acids / Nanomedicine: an integrative approach, NANOMED Ghent University
Nanomedicine (NM) is regarded as one of the most promising applications of nanotechnology, as it would allow the development of tailored therapies, with a high level of selectivity and efficacy. Although much research has been performed over the past deca
Peptide Hydrogels are swollen networks of self-assembled peptide nanofibers that contain large
amounts of water. An important characteristic of such hydrogels is that they can be aspirated and
injected through a standard needle. Recently, we developed such peptide hydrogels that extended
the pain killing effect of morphine form several hours up to 3-4 days. However, further extension of
the sustained release time is ...
Unravelling the cellular response to photoporation Ghent University
Delivering compounds into cells is a ubiquitous requirement for fundamental life science research
and cell-based clinical applications. Since cells are protected from the outside world by their plasma
membrane, it requires sophisticated technology to deliver compounds across this barrier without
causing toxicity. Photoporation is emerging as a powerful technology to achieve exactly this. It relies
on laser illumination of ...
Developing a preclinical test platform that incorporates interpatient variability to lower the attrition rate in antimicrobial drug development Ghent University
The high attrition rate in drug development is a major issue for the pharmaceutical industry. Of particular concern is the high failure rate in clinical phase 2 and 3. At this point, failure is disastrous from an economic point of view, leading to an outrageous general cost to bring a compound to the market. Providing predictive in vitro tests in a preclinical stage is generally acknowledged to lower the chances of failure in clinical phases. ...
Novel heterocyclic building blocks for highly functionalized cyclopentanoid scaffolds Ghent University
Natural product-inspired drug design is a key and time-tested strategy in the development of new medicines. Nature produces an astounding number of highly complex molecules, each of which has specific functions important for the survival of the producing species. As function follows from structure, the various substructures found in the small molecules produced by living organisms (so-called metabolites) are important leads in the molecular ...
Function and post-translational regulation of the PP2A methylesterase PME-1 in the cellular response to stress conditions KU Leuven
Cells have developed diverse mechanisms to protect themselves from acute damage by environmental stresses, such as nutrient or growth factor deprivation, oxidative stress and DNAdamage. In all these responses, stress-regulated protein kinases and phosphatases are commonly involved. Protein Phosphatase 2A (PP2A) is a major cellular phosphatase with pleiotropic functions, including in stress signaling. Here, we aim to investigate how cellular ...
The evaluation of nanomaterials-mediated induction of immunogenic cell death for tumor therapy KU Leuven
Conventional cancer treatment, encompassing either radiation, surgery or chemotherapy is insufficient to treat all tumor types. Novel methods are being explored, among which immunotherapy (aimed at activating the immune system to detect and destroy tumor cells) and nanomedicine (the use of nanosized materials (NMs) for the targeted delivery of therapeutics) are receiving great interest. We have demonstrated that by finely tuning NM ...
Discovery and mechanism of action of novel hEag1 potassium channel lead molecules with anti-cancer activity. KU Leuven
Cancer remains an important cause of mortality and economic losses worldwide. For an improved cancer therapy, a permanent need for the discovery of new-generation, refined anticancer agents is needed. Exploitation of genuinely novel classes of cancer targets with new mechanisms of action provides great opportunities for the discovery of new anticancer drugs. Targeting the voltage-gated potassium channel hEag1 in cancer is one of the most ...