Title Participants Abstract "Educational impact of discussing results among nuclear medicine physicians participating in interobserver reproducibility studies : our experience with I-123 FP-CIT SPECT" "Marianne Tondeur, Anne-Sophie Hambye, Alain San, Hamphrey Ham" "Objectives In nuclear medicine, reproducibility studies allow the assessment of the robustness of a technique. They could also be used in continuing education. This study evaluates this educational impact. Methods Two series of clinical data and I-123 fluoropropyl-carbomethoxy-iodophenyl-nortropane single photon emission tomography images from 12 patients were prepared before initiating the study. Each series covered similarly a wide spectrum of clinical situations and images. Nuclear medicine physicians having experience with I-123 fluoropropyl-carbomethoxy-iodophenyl-nortropane single photon emission tomography were recruited and assigned two similar groups regarding their level of experience and type of institutions from which they were issued. Clinical data and images were transmitted by e-mail. For each case, observers had to choose among three answers: normal, equivocal, and abnormal. Answers were returned anonymously. Before the second series was sent, observers from group A had a collegiate discussion on the results of the first series of cases. Observers from group B received the second series without any information regarding analysis of series 1. Results In group A, median agreement increased from 75% (series 1) to 100% (series 2); in group B it was 75% (series 1) and 87% (series 2). In group A, a 100% agreement was observed for four cases (series 1) and for eight cases (series 2). In group B, a 100% agreement was observed for four cases for both series. Conclusion These preliminary results, obtained in a small sample of observers, suggest that participating and discussing results of interobserver reproducibility studies seems to have a positive educational impact and therefore improves interobserver reproducibility. Nucl Med Commun 32:410-415 (C) 2011 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins." "Standardisation of EGFR FISH in colorectal cancer: results of an international interlaboratory reproducibility ring study (vol 65, pg 218, 2012)" "A Sartore-Bianchi, Steffen Fieuws, S Veronese" "Standardisation of EGFR FISH in colorectal cancer: results of an international interlaboratory reproducibility ring study" "Steffen Fieuws, Sara Vander Borght, Sabine Tejpar" "Epidermal growth factor receptor (EGFR) gene copy number evaluated by fluorescence in situ hybridisation (FISH) can discriminate among KRAS wild-type patients those with better outcome to EGFR-targeted therapy in metastatic colorectal cancer, further enhancing selection of patients. Nevertheless, enumeration of gene copies is challenging and the lack of analytical standardisation has limited incorporation of the test into the clinical practice. We therefore assessed EGFR FISH interlaboratory consensus among five molecular diagnostic reference centres." "A multicenter study to assess the reproducibility of antiphospholipid antibody results produced by an automated system" "Katrien Devreese, A Poncet, E Lindhoff-Last, J Musial, P de Moerloose, P Fontana" "The reproducibility of statistical results in psychological research: An investigation using unpublished raw data." "Richard Artner, Frits Traets, Francis Tuerlinckx, Wolf Vanpaemel" "We investigated the reproducibility of the major statistical conclusions drawn in 46 articles published in 2012 in three APA journals. After having identified 232 key statistical claims, we tried to reproduce, for each claim, the test statistic, its degrees of freedom, and the corresponding p value, starting from the raw data that were provided by the authors and closely following the Method section in the article. Out of the 232 claims, we were able to successfully reproduce 163 (70%), 18 of which only by deviating from the article's analytical description. Thirteen (7%) of the 185 claims deemed significant by the authors are no longer so. The reproduction successes were often the result of cumbersome and time-consuming trial-and-error work, suggesting that APA style reporting in conjunction with raw data makes numerical verification at least hard, if not impossible. This article discusses the types of mistakes we could identify and the tediousness of our reproduction efforts in the light of a newly developed taxonomy for reproducibility. We then link our findings with other findings of empirical research on this topic, give practical recommendations on how to achieve reproducibility, and discuss the challenges of large-scale reproducibility checks as well as promising ideas that could considerably increase the reproducibility of psychological research. (PsycInfo Database Record (c) 2020 APA, all rights reserved)." "Reproducibility of the Kinematics in Rotational High-Velocity, low-amplitude thrust of the upper cervical spine: a cadaveric study" "Silvia Gianola, Erik Cattrysse, Steven Provyn, Peter Van Roy" "Objective: this study aimed to investigate the reproducibility of the kinematics in rotational high-velocity, low-amplitude (HVLA) thrust of the upper cervical spine. Methods: twenty fresh human cervical specimens were studied in a test-retest situation with 2 manual therapists. Kinematics of C1-C2 and C0-C1 were examined during segmental rotational HVLA manipulation through an ultrasound-based tracking system. The thrust moment was analyzed by 3-dimensional aspects: the range of motion of axial rotation, flexion-extension, lateral banding, and the cross-correlation between the axial rotation and the coupled lateral banding components. Results: during rotational HVLA thrust on C1-C2, the main axial rotation demonstrates an intraexaminer relationship varying from almost perfect to fair (intraclass correlation coefficient = 0.71; intraclass correlation coefficient = 0.35) and a substantial interexaminer correlation of 0.73. Conclusions: this study showed substantial levels of reliability for the main axial rotation component of segmental manual rotational HVLA thrust on C1-C2. Intra- and interrater reliability for flexion-extension, lateral bending, and cross-correlation was low. (J. Manipulative Physiol. Ther. 2014, xx: 1-8)." "Kinetic modeling and long-term test-retest reproducibility of the mGluR5 PET tracer 18F-FPEB in human brain" "Gil Leurquin-Sterk, Andrey Postnov, Bart de Laat, Cindy Casteels, Sofie Celen, Guy Bormans, Michel Koole, Koen Van Laere" "(18)F-FPEB is a promising PET tracer for studying the metabotropic glutamate subtype 5 receptor (mGluR5) expression in neuropsychiatric disorders. To assess the potential of (18)F-FPEB for longitudinal mGluR5 evaluation in patient studies, we evaluated the long-term test-retest reproducibility using various kinetic models in the human brain. Nine healthy volunteers underwent consecutive scans separated by a 6-month period. Dynamic PET was combined with arterial sampling and radiometabolite analysis. Total distribution volume (V(T)) and nondisplaceable binding potential (BP(ND)) were derived from a two-tissue compartment model without constraints (2TCM) and with constraining the K(1)/k(2) ratio to the value of either cerebellum (2TCM-CBL) or pons (2TCM-PONS). The effect of fitting different functions to the tracer parent fractions and reducing scan duration were assessed. Regional absolute test-retest variability (aTRV), coefficient of repeatability (CR) and intraclass correlation coefficient (ICC) were computed. The 2TCM-CBL showed best fits. The mean 6-month aTRV of V(T) ranged from 8 to 13% (CR  0.6 for all kinetic models. BPND from 2TCM-CBL with a sigmoid fit for the parent fractions showed the best reproducibility, with aTRV ≤ 7% (CR  0.9 in most regions. Reducing the scan duration from 90 to 60 min did not affect reproducibility. These results demonstrate for the first time that (18)F-FPEB brain PET has good long-term reproducibility, therefore validating its use to monitor mGluR5 expression in longitudinal clinical studies. We suggest a 2TCM-CBL with fitting a sigmoid function to the parent fractions to be optimal for this tracer." "Reproducibility of 20-min Time-trial Performance on a Virtual Cycling Platform" "Guilherme Matta, Andrew Edwards, Bart Roelands, Florentina Hettinga" "This study aimed to analyse the reproducibility of mean power output during 20-min cycling time-trials, in a remote home-based setting, using the virtual-reality cycling software, Zwift. Forty-four cyclists (11 women, 33 men; 37±8 years old, 180±8 cm, 80.1±13.2 kg) performed 3×20-min time-trials on Zwift, using their own setup. Intra-class correlation coefficient (ICC), coefficient of variation (CV) and typical error (TE) were calculated for the overall sample, split into 4 performance groups based on mean relative power output (25% quartiles) and sex. Mean ICC, TE and CV of mean power output between time-trials were 0.97 [0.95-0.98], 9.4 W [8.0-11.3 W], and 3.7% [3.2-4.5], respectively. Women and men had similar outcomes (ICC: 0.96 [0.89-0.99] vs. 0.96 [0.92-0.98]; TE: 8.3 W [6.3-13.1] vs. 9.7 W [8.2-12.2]; CV: 3.8% [2.9-6.1] vs. 3.7% [3.1-4.7], respectively), although cyclists from the first quartile showed a lower CV in comparison to the overall sample (Q1: 2.6% [1.9-4.1] vs. overall: 3.7% [3.2-4.5]). Our results indicate that power output during 20-min cycling time-trials on Zwift are reproducible and provide sports scientists, coaches and athletes, benchmark values for future interventions in a virtual-reality environment." "Repeatability and reproducibility of ADC measurements" "Nicolas F Michoux, Jakub Wladyslaw Ceranka, Jef Vandemeulebroucke, Frank Peeters, Pierre Lu, Julie Absil, Perrine Triqueneaux, Laurence Collette, Inneke Willekens, Carola Brussaard, Olivier Debeir, Stephan Hahn, Hubert Raeymaekers, Johan De Mey, Thierry Metens, Frédéric E Lecouvet" "OBJECTIVES: Multicenter oncology trials increasingly include MRI examinations with apparent diffusion coefficient (ADC) quantification for lesion characterization and follow-up. However, the repeatability and reproducibility (R&R) limits above which a true change in ADC can be considered relevant are poorly defined. This study assessed these limits in a standardized whole-body (WB)-MRI protocol.METHODS: A prospective, multicenter study was performed at three centers equipped with the same 3.0-T scanners to test a WB-MRI protocol including diffusion-weighted imaging (DWI). Eight healthy volunteers per center were enrolled to undergo test and retest examinations in the same center and a third examination in another center. ADC variability was assessed in multiple organs by two readers using two-way mixed ANOVA, Bland-Altman plots, coefficient of variation (CoV), and the upper limit of the 95% CI on repeatability (RC) and reproducibility (RDC) coefficients.RESULTS: CoV of ADC was not influenced by other factors (center, reader) than the organ. Based on the upper limit of the 95% CI on RC and RDC (from both readers), a change in ADC in an individual patient must be superior to 12% (cerebrum white matter), 16% (paraspinal muscle), 22% (renal cortex), 26% (central and peripheral zones of the prostate), 29% (renal medulla), 35% (liver), 45% (spleen), 50% (posterior iliac crest), 66% (L5 vertebra), 68% (femur), and 94% (acetabulum) to be significant.CONCLUSIONS: This study proposes R&R limits above which ADC changes can be considered as a reliable quantitative endpoint to assess disease or treatment-related changes in the tissue microstructure in the setting of multicenter WB-MRI trials.KEY POINTS: • The present study showed the range of R&R of ADC in WB-MRI that may be achieved in a multicenter framework when a standardized protocol is deployed. • R&R was not influenced by the site of acquisition of DW images. • Clinically significant changes in ADC measured in a multicenter WB-MRI protocol performed with the same type of MRI scanner must be superior to 12% (cerebrum white matter), 16% (paraspinal muscle), 22% (renal cortex), 26% (central zone and peripheral zone of prostate), 29% (renal medulla), 35% (liver), 45% (spleen), 50% (posterior iliac crest), 66% (L5 vertebra), 68% (femur), and 94% (acetabulum) to be detected with a 95% confidence level." "Reproducibility and symptomatic predictors of a slow nutrient drinking test in health and in functional dyspepsia" "Sébastien Kindt, B Coulie, E Wajs, J Janssens, J Tack" "Impaired accommodation to a meal has been recognized as a pathophysiological mechanism in functional dyspepsia (FD). Based on observations in tertiary care patients, the drinking test has been proposed as a non-invasive tool to estimate accommodation. Our aim was to assess the reproducibility of the drinking test and its correlation with demographic, symptomatic and pathophysiological parameters in secondary care FD patients and healthy controls. Thirty-four healthy controls and 78 FD patients completed a drinking test (3 respectively 2 times), a gastric emptying study and an FD symptom questionnaire. Factors influencing maximal volume and gastric emptying were determined, and the reproducibility of the drinking test was investigated. The maximal satiety was reached at a lower volume in patients (489 +/- 276 and 503 +/- 248 mL for first and second test respectively vs 937 +/- 428 and 1048 +/- 421 mL, P < 0.0001). The ingested amount depended on age, sex and baseline FD symptom score. Patients' sex, final satiety score, total score for stomach complaints at screening and total symptom score before test accounted for the total symptom score after the test. The slow nutrient drinking test confirms its possible role as an attractive non-invasive and reproducible tool for the diagnosis of impaired accommodation and for the assessment of treatment responsiveness."