Researcher

Sales Ibiza Martinez

Research Expertise:My initial academic training was in Pharmacy, where I learned about principles related to the industry, manufacture of medicine, and human physiology. After this stage, I focused on Immunology and scientific research in order to complete my background in pharmacy. During my PhD research, I studied the role of Nitric Oxide in lymphocyte T activation after TCR engagement by antigen presentation cells. Since receiving my PhD in 2009, I have had the opportunity to work on a project related to innate lymphoid cell (ILC) immune responses in the gut. Specifically, my main work was focused in learning how ILCs perceive, integrate, and respond to environmental cues (such as microbiota) to exert their function on a novel multicellular unit orchestrated by neurotrophic factors.

Keywords:ENTERIC NERVOUS SYSTEM, NEURODEGENERATION, GALT-ENTERIC NERVOUS SYSTEM-ENTEROCYTES, IMMUNE RESPONSE, INFLAMMATORY BOWEL DISEASE, Veterinary medicine

Disciplines:Gastro-enterology, Applied immunology, Inflammation, Innate immunity, Cell growth and development, Cell signalling, Cellular interactions and extracellular matrix, Developmental biology, Stem cell biology, Histology, Neurophysiology

Research techniques:1. Cellular techniques and associated instruments Cell Culture: Isolation and characterization of aortic endothelial cells from human (HUVEC); Transfection. Differentiation of T lymphocytes towards Th1, Th2 subpopulations. Isolation cells from the gut, Lamina propria, Pilling Muscularis. Organoid: of glial and Neurons from embrionary gut; epithelial cells. 2. Molecular biology laboratory techniques and associated instruments DNA and RNA isolation and purification; RT-PCR (standard and real time); electrophoresis (agarose, DNA, RNA); restriction enzyme digestion; site-directed mutagenesis; DNA sequence analysis software; ribosomal IP; BLAST, FASTA and Gene Expression searches; cloning (plasmid construction and isolation, E. coli transformation); transcription-translation in vitro; protein isolation and quantification; Western-blot, Fluorescence and Confocal Microscopy; Flow cytometry-Symphony, FACS-Sorter etc. 3. Mice handling DNA, RNA, cells and protein isolation from tissues, ribosomal IP. Intravital microscopy in mice and rat. Isolation cells from the adult gut and embryonic guts or lymphoid nodes. Administration and infection via oral, peritoneal, subcutaneous, and ocular. Transgenic mice breeding.