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Researcher

Nina Hermans

  • Research Expertise:*ANALYSIS OF BIOMARKERS OF IN VIVO OXIDATIVE - ANTIOXIDANT STATUS (matrix: plasma, urine, cells, tissue samples; much expertise with studies in animal models, as well as in conducting clinical trials) - Glutathione (GSH) - Glutathione peroxidase (GPx) - Fat-soluble vitamins (vitamin E, A, Beta-carotene) - Co-enzyme Q - Oxidized LDL (OxLDL) - Malondialdehyde (MDA) -8-OHdG - Selenium *GIDM-COLON MODEL: STUDIES OF BIOTRANSFORMATION AND ABSORPTION OF BIOACTIVE COMPOUNDS (e.g. polyphenols, alkaloids, vitamins ....) IN A VALIDATED GASTRO-INTESTINAL SIMULATION MODEL, GIDM-COLON *ANALYSIS OF MINERALS AND TRACE ELEMENTS (matrix: plasma, urine, cells, tissue samples, foods, food supplements, drugs, raw materials)
  • Keywords:NUTRITION, ANALYSIS MINERALS, OXIDATIVE STRESS, ANTIOXIDATIVE ACTIVITY, METABOLOMICS, POLYPHENOLS, OVERWEIGHT AND OBESITY, Pharmacy and pharmacology
  • Disciplines:Metabolomics, Metabolic diseases, Vascular diseases, Nutrition and dietetics, Biomarker discovery and evaluation, Drug discovery and development, Clinical trials, Nutraceuticals, Pharmacognosy and phytochemistry
  • Research techniques:-In vivo antioxidant research: validated methods to assess biomarkers of oxidative stress in biological fluids (urine, plasma): assessment of 8-OHdG, OxLDL (Elisa), malondialdehyde (HPLC-fluorescence detection), fat-soluble vitamins, coenzyme Q10, glutathione (HPLC-Coularray detection), glutathione peroxidase (UV/VIS spectrophotometry) - Laboratory animal model (rat) of oxidative stress -Battery of in vitro antioxidant assays : DPPH, FRAP, microsomal lipid peroxidation assays (UV/VIS spectrophotometry). -GIDM-COLON model: in vitro simulation model of the human gastro-intestinal tract (stomach, small intestine and colon phase) -Analysis and identification of metabolites (following GIDM-COLON experiments): HPLC-UV/DAD; HPLC-QTOF-MS -Atomic Absorption Spectrophotometry (Flame AAS) to conduct analyses of minerals.
  • Users of research expertise:Pharmaceutical companies and food industry, Nubel Research groups interested in the relationship between certain (chronic) pathologies and oxidative stress. Research groups or companies (pharmaceutical or food/food supplement companies) interested in the in vitro or in vivo antioxidant evaluation of a synthetic compound or plant-derived compound/extract.