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Researcher

Elise Van Breedam

  • Research Expertise:Development and characterization of a 3D neurospheroid model for ischemic stroke research. In this context, I gained experience in: (1) 2D and 3D cell culture (incl. stem cells, neural cells and primary monocytes, microglia(-progenitors)) (2) differentiation of induced pluripotent stem cells to neural stem cells, and further differentiation into neurons and astrocytes, (3) development of reporter cell lines, by means of lentiviral vector transduction, (4) in vitro luminescent assays to monitor neurospheroid viability and growth, (5) execution of hypoxia experiments on 2D and 3D cell cultures, (6) flow cytometry of dissociated neurospheroids, (7) immunocytochemical stainings and microscopic imaging of neurospheroids in semi-high throughput, (8) ELISA to detect cytokines produced by neurospheroids.
  • Keywords:HYPOXIA, IN VITRO MODEL, BRAIN ORGANOIDS, ISCHEMIA, NEUROINFLAMMATION, STEM CELLS, 3D CELL CULTURE, STROKE, NEUROSPHEROIDS, Medicine
  • Disciplines:Hematology, Molecular and cell biology, Neurosciences, Inflammation, Stem cell biology, Tissue engineering, In vitro testing
  • Research techniques:2D and 3D cell culture - immunocytochemistry (in semi-high throughput for neurospheroids) - microscopy - in vitro luminescence assays - flow cytometry - ELISA - lentiviral vector transduction - hypoxia experiments
  • Users of research expertise:(Bio)medical researchers interested in 3D neural cell culture and/or hypoxia-experiments, for example in context of ischemic stroke studies.