Researcher

Debbie Le Blon

Research Expertise:Cell cultures and assays for both murine and human primary immune cells (including T-cells, macrophages and dendritic cells) and stem cells (including embryonic, induced pluripotent, mesenchymal and neural stem cells). Ex vivo genetic modification of cell populations by mRNA, DNA plasmids, transposons and lentiviral vectors. Multiple color flow cytometry for cell phenotyping. Multiple color immunocytochemistry analysis for cell phenotyping. Experimental animal models for neuro-inflammation (including the EAE and cuprizone mouse models for multiple sclerosis). Cerebral and spinal engraftment of stem cells in mice. Generation of bone marrow chimeric mice. Multiple color and quantitative analysis of immunofluorescence microscopy for the analysis of neuro-inflammation.

Disciplines:Inflammation, Transplantation immunology, Neurological and neuromuscular diseases

Research techniques:Cell cultures and assays Ex vivo genetic modification of mammalian cells by lentiviral transduction, electroporation, lipofection. Multiple color flow cytometry. Multiple color immunocytochemistry. Experimental animal models for neuro-inflammation (including the EAE and cuprizone mouse models for multiple sclerosis). Cerebral and spinal engraftment of stem cells in mice. Generation of bone marrow chimeric mice. Multiple color and quantitative analysis of immunofluorescence microscopy.

Users of research expertise:Researchers interested in mouse models for neuro-inflammation, and analysis of neuroimmunology both in vivo and in vitro.