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- Research interest:- Prolyl oligopeptidase in the central nervous system and prolyl oligopeptidase inhibitors: new therapeutic strategies in neurodegenerative disease and Carboxypeptidase M: substrates and interaction partners in health and disease (kidney, adipocytes).- Eiwit engineering en structuurgebaseerde ontwikkeling van geneesmiddelen: aspecten van enzymologie, eiwitchemie en moleculaire biologie.- Know-how in enzymology: research on catalytic mechanisms, kinetics and characterisation of enzyme inhibitors. Enzymes currently under investigation: prolyl oligopeptidase, carboxypeptidase M, dipeptidyl peptidase 4 (porphyromonas gingivalis). Other enzymes: proline specific peptidases, serine proteases of the coagulation and fibrinolysis systems, glucose isomerase, amylases and glucoamylases, glycolytic enzymes (such as triosephosphate isomerase, glyceraldehydephosphate dehydrogenase, glycerol kinase, hexokinase), thiolases, glutathionyl-spermidine synthase, peroxidases, prostaglandin H synthase (COX-1).-
- Keywords:ENZYME INHIBITORS, ENZYME-ASSAYS, PROLYLOLIGOPEPTIDASES, CARBOXYPEPTIDASE M, PEPTIDASE, ENZYME SUBSTRATES, Pharmacy and pharmacology
- Disciplines:Medicinal and biomolecular chemistry, Biochemistry and metabolism, Molecular and cell biology, Plant biology, Systems biology, Biophysics, Medical biochemistry and metabolism, Biomarker discovery and evaluation, Drug discovery and development, Medicinal products, Pharmaceutics, Pharmacognosy and phytochemistry, Pharmacology, Pharmacotherapy, Toxicology and toxinology, Other pharmaceutical sciences
- Research techniques:- Ultracentrifugation: Optima MAX-XP (Bechman Coulter).- Protein purification and characterisation. Equipment: Äkta Prime plus and Äkta explorer (GE Healthcare), HPLC. Equipment for electrophoresis and western blots (OptiGo-750 Optivex Isogen Life Science gel documentation system).- Enzyme kinetic measurements using absorption, fluorescence or chemiluminescence read-outs. Equipment: Spectramax Plus (Molecular devices), Versamax (Molecular Devices), TECAN infinite M200 microtiterplate readers.
- Users of research expertise:- Pharmaceutical industry- Biotech companies- Clinical Research Laboratories - Research groups- Government