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Sputum is a surrogate for bronchoalveolar lavage for monitoring Mycobacterium tuberculosis transcriptional profiles in TB patients

Journal Contribution - Journal Article

Pathogen-targeted transcriptional profiling in human sputum may elucidate the physiologic state of Mycobacterium tuberculosis (M. tuberculosis) during infection and treatment. However, whether M. tuberculosis transcription in sputum recapitulates transcription in the lung is uncertain. We therefore compared M. tuberculosis transcription in human sputum and bronchoalveolar lavage (BAL) samples from 11 HIV-negative South African patients with pulmonary tuberculosis. We additionally compared these clinical samples with in vitro log phase aerobic growth and hypoxic non-replicating persistence (NRP-2). Of 2179 M. tuberculosis transcripts assayed in sputum and BAL via multiplex RT-PCR, 194 (8.9%) had a p-value

Journal: Tuberculosis (Edinburgh, Scotland)
ISSN: 1472-9792
Volume: 100
Pages: 89-94
Number of pages: 6
Publication year:2016
Keywords:Mycobacterium tuberculosis/genetics, Mycobacterium tuberculosis/physiology, Sputum/microbiology, Bronchoalveolar lavage/microbiology, Computational biology/methods, * Gene expression profiling, Tuberculosis, pulmonary/epidemiology/*microbiology, GENE-EXPRESSION, ADAPTATION, PERSISTENCE, Cardiovascular & respiratory medicine, Immunology, Microbiology