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Semi-invasive, non-contact measurements of chicken embryo heart rate using video imaging and signal processing

Book Contribution - Book Chapter Conference Contribution

The chicken embryo provides an excellent model organism for physiological and developmental biology studies. The chick chorioallantoic membrane (CAM) is widely used to study angiogenesis and vasculogenesis in primary tumour growth. The cardiovascular system is the first organ system to form and function in the developing embryo. Heart rate is deemed to be an important physiological parameter in such studies. The heart rate of a developing embryo can be very informative in developmental studies of cardiac rhythm. Many studies have investigated the development of techniques to measure avian embryonic heart rate from incubated eggs. However, the existing techniques disturb the incubation process and/or are sensitive to embryonic motion. A novel non-contact, semi-invasive, and motion-tolerant technique for measuring embryonic heart rate from chicken eggs using video imaging and signal processing is described and implemented in this paper. The method was developed using videos captured from 30 eggs during incubation. Heart rate was estimated using frequency analysis techniques and was in agreement with results from previous studies. The method proposed in this paper provides a real-time approach to monitoring embryonic heart rate during the development of the embryo; in addition it can provide a promising technique for monitoring the developing vasculature in primary tumour growth.
Book: Precision Livestock Farming '13
Pages: 846 - 855
ISBN:978-90-8826-333-0
Publication year:2013