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Rice microtubuleU+2010associated protein IQ67U+2010DOMAIN14 regulates rice grain shape by modulating microtubule cytoskeleton dynamics

Journal Contribution - Journal Article

Cortical microtubule (MT) arrays play a critical role in plant cell shape determination by defining the direction of cell expansion. As plants continuously adapt to everU+2010changing environmental conditions, multiple environmental and developmental inputs need to be translated into changes of the MT cytoskeleton. Here, we identify and functionally characterize an auxinU+2010inducible and MTU+2010localized protein OsIQ67U+2010DOMAIN14 (OsIQD14), which is highly expressed in rice seed hull cells. We show that while deficiency of OsIQD14 results in short and wide seeds and increases overall yield, overexpression leads to narrow and long seeds, caused by changed MT alignment. We further show that OsIQD14U+2010mediated MT reordering is regulated by specifically affecting MT dynamics, and ectopic expression of OsIQD14 in Arabidopsis could change the cell shape both in pavement cells and hypocotyl cells. Additionally, OsIQD14 activity is tightly controlled by calmodulin proteins, providing an alternative way to modify the OsIQD14 activity. Our results indicate that OsIQD14 acts as a key factor in regulating MT rearrangements in rice hull cells and hence the grain shape, and allows effective local cell shape manipulation to improve the rice yield trait.
Journal: PLANT BIOTECHNOLOGY JOURNAL
ISSN: 1467-7652
Issue: 5
Volume: 18
Pages: 1141 - 1152
BOF-keylabel:yes
IOF-keylabel:yes
BOF-publication weight:10
Authors:International
Authors from:Government
Accessibility:Open