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Publication
Reusable chromium-coated quartz crystal microbalance for immunosensing
Journal Contribution - Journal Article
The application of oxidized chromium as a reusable platform for the development of immunosensors is presented. Chromium films were deposited on quartz crystal microbalances to study the affinity interaction between rabbit immunoglobulin G (IgG) and goat anti-rabbit IgG. A covalent approach, based on the silane chemistry, was followed for the grafting of either the rabbit IgG or the anti-rabbit IgG on the silane-modified chromium surface. Next the differences between the deposition of rabbit IgG on immobilized anti-rabbit IgG and the deposition of anti-rabbit IgG on immobilized rabbit IgG were investigated. The chromium layer could be reused between experiments, after the proper removal of the organic layers with piranha etch, obtaining a high repeatability in the steps of the functionalization protocol. (C) 2011 Elsevier B.V. All rights reserved.
Journal: COLLOIDS AND SURFACES B-BIOINTERFACES
ISSN: 0927-7765
Issue: 1
Volume: 88
Pages: 191 - 195
Publication year:2011
Keywords:Oxidized chromium, Immunosensors, Quartz crystal microbalance
BOF-keylabel:yes
IOF-keylabel:yes
BOF-publication weight:1
CSS-citation score:1
Authors:International
Authors from:Government, Higher Education, Private
Accessibility:Open