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Quantitative Peptidomics with Isotopic and Isobaric Tags

Book Contribution - Chapter

In differential peptidomics, peptide profiles are compared between biological samples and the resulting expression levels are correlated to a phenotype of interest. This, in turn, allows us insight into how peptides may affect the phenotype of interest. In quantitative differential peptidomics, both label-based and label-free techniques are often employed. Label-based techniques have several advantages over label-free methods, primarily that labels allow for various samples to be pooled prior to liquid chromatography-mass spectrometry (LC-MS) analysis, reducing between-run variation. Here, we detail a method for performing quantitative peptidomics using stable amine-binding isotopic and isobaric tags.
Book: Peptidomics: Methods and Strategies
Series: Methods in Molecular Biology
Pages: 141 - 159
Publication year:2018
Keywords:peptidomics, isotopic tags, isobaric tags, mass spectrometry, protocols