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The presence of extracellular microRNAs in the media of cultured Drosophila cells

Journal Contribution - e-publication

While regulatory RNA pathways, such as RNAi, have commonly been described at an intracellular level, studies investigating extracellular RNA species in insects are lacking. In the present study, we demonstrate the presence of extracellular microRNAs (miRNAs) in the cell-free conditioned media of two Drosophila cell lines. More specifcally, by means of quantitative real-time PCR (qRT-PCR), we analysed the presence of twelve miRNAs in extracellular vesicles (EVs) and in extracellular Argonaute-1 containing immunoprecipitates, obtained from the cell-free conditioned media of S2 and Cl.8 cell cultures. Next-generation RNA-sequencing data confrmed our qRT-PCR results and provided evidence for selective miRNA secretion in EVs. To our knowledge, this is the frst time that miRNAs have been identifed in the extracellular medium of cultured cells derived from insects, the most speciose group of animals.
Journal: Scientific Reports
ISSN: 2045-2322
Issue: 1
Volume: 8
Publication year:2018
BOF-publication weight:2
CSS-citation score:1
Authors from:Higher Education