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Phylogenetic utility of the AP3/DEF K-domain and its molecular evolution in Impatiens (Balsaminaceae)

Journal Contribution - Journal Article

Subtitle:Molecular Phylogenetics and Evolution
APETALA3 (AP3)/DEFICIENS (DEF) is a MADS-box transcription factor that is involved in establishing the identity of petal and stamen floral organs. The AP3/DEF gene lineage has been extensively examined throughout the angiosperms in order to better understand its role in floral diversity and evolution. As a result, a large number of cloned AP3/DEF orthologues are available, which can be used for the design of taxon specific primers for phylogeny reconstruction of close relatives of the group of interest. Following this reasoning, we investigated the phylogenetic utility of the two AP3/DEF paralogues (ImpDEF1 and ImpDEF2) that were recently identified in the genus Impatiens (Balsaminaceae). K-domain introns 4 and 5 of both AP3/DEF duplicates were amplified and sequenced for 59 Impatiens species. Phylogenetic analyses of the separated and combined ImpDEF1 and ImpDEF2 data sets result in highly congruent topologies with the previously obtained chloroplast atpB-rbcL data set. Combination of chloroplast and nuclear matrices results in a well-supported evolutionary hypothesis of Impatiens. Our results show that introns 4 and 5 in AP3/DEF-like genes are a valuable source of characters for phylogenetic studies at the infrageneric level. © 2006 Elsevier Inc. All rights reserved.
Journal: Molecular phylogenetics and evolution
ISSN: 1055-7903
Issue: 1
Volume: 43
Pages: 225-239
Publication year:2007
Keywords:APETALA3/DEFICIENS, Gene duplication, Impatiens, ImpDEF1, ImpDEF2, K-domain, Phylogenetic utility, chloroplast DNA, deficiens protein, primer DNA, amino acid sequence, article, Bayes theorem, biological model, comparative study, DNA sequence, gene dosage, genetic variability, genetics, intron, molecular evolution, molecular genetics, nucleotide sequence, phylogeny, species difference, Amino Acid Sequence, Base Sequence, Bayes Theorem, DEFICIENS Protein, DNA Primers, DNA, Chloroplast, Evolution, Molecular, Gene Dosage, Introns, Models, Genetic, Molecular Sequence Data, Phylogeny, Sequence Analysis, DNA, Species Specificity, Variation (Genetics), Balsaminaceae, Magnoliophyta