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Multi-parametric screening in Arabidopsis thaliana seedlings

Book Contribution - Chapter

Phenotypic screening and subsequent target identification approaches are very valuable to identify chemical probes that can be used to explore the connection between phenotypes and biological pathways. However, assessing a phenotypic effect in plants in a high-throughput fashion is a challenging task and often requires expensive readout devices. In this chapter, we describe a cost-effective multi-parametric screening procedure that is compatible with liquid-handling systems and that enables the assessment of phenotypes in Arabidopsis thaliana seedlings in an automated way.
Book: Plant chemical genomics : methods and protocols
Series: Methods in Molecular Biology
Pages: 1 - 7
Publication year:2018