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Expression of immune-related genes in rectum and colon descendens of Irritable Bowel Syndrome patients is unrelated to clinical symptoms

Journal Contribution - Journal Article

Background: Mucosal immune activation has been postulated to play an importantrole in the pathogenesis of irritable bowel syndrome (IBS). However, data are conflicting and often based on small patient cohorts. Here, we aimed to evaluate thegene expression of a large set of immune‐related genes in mucosal biopsies from IBSpatients and healthy volunteers (HV).Methods: A total of 171 IBS patients and 127 HV were included in the study. Rectumbiopsies were collected from a cohort of 70 HV and 77 IBS patients (Rome III) andcolon descendens biopsies from another cohort of 57 HV and 94 IBS patients (RomeII). Gene expression was assessed using OpenArray technology, and validated questionnaires were used to evaluate clinical characteristics (GI symptoms, somatization,anxiety, and depression).Key Results: A subset of IBS patients (33%) with increased immune activation in thecolon descendens was identified using multivariate analysis and displayed increasedgene expression of IL1B (3‐fold change), prostaglandin synthase PTGS2 (2.1‐foldchange), and the G‐protein‐coupled receptor MRGPRX2 (10.7‐fold change). Clinicalcharacteristics in this subgroup were however similar to the rest of the patient cohort. Analysis of rectal biopsies failed to identify such subgroup of “immuno‐active”IBS patients in the other patient cohort.Conclusion: A subset of IBS patients reveals evidence of immune activation in thecolon descendens, but not in the rectum; however, gene expression is unrelated toclinical symptoms. To what extent this subgroup might however respond to anti‐inflammatory therapy remains to be investigated.
Journal: Neurogastroenterology and Motility
ISSN: 1350-1925
Issue: 6
Volume: 31
Pages: 1 - 10
BOF-keylabel:yes
IOF-keylabel:yes
BOF-publication weight:2
Authors:International
Authors from:Government, Higher Education
Accessibility:Open