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Endospore Appendages: a novel pilus superfamily from the endospores of pathogenic Bacilli

Journal Contribution - Journal Article

Bacillus cereus sensu lato is a group of Gram-positive endospore-forming bacteria with high ecological diversity. Their endospores are decorated with micrometer-long appendages of unknown identity and function. Here, we isolate endospore appendages (Enas) from the food poisoning outbreak strain B. cereus NVH 0075-95 and find proteinaceous fibers of two main morphologies: S- and L-Ena. By using cryoEM and 3D helical reconstruction of S-Enas, we show these to represent a novel class of Gram-positive pili. S-Enas consist of single domain subunits with jellyroll topology that are laterally stacked by β-sheet augmentation. S-Enas are longitudinally stabilized by disulfide bonding through N-terminal connector peptides that bridge the helical turns. Together, this results in flexible pili that are highly resistant to heat, drought, and chemical damage. Phylogenomic analysis reveals a ubiquitous presence of the ena-gene cluster in the B. cereus group, which include species of clinical, environmental, and food importance. We propose Enas to represent a new class of pili specifically adapted to the harsh conditions encountered by bacterial spores.

Journal: EMBO Journal
ISSN: 0261-4189
Issue: 17
Volume: 40
  • WoS Id: 000653609700001
  • Scopus Id: 85106246567
  • DOI: https://doi.org/10.15252/embj.2020106887
  • ORCID: /0000-0002-9775-4102/work/99485975
  • ORCID: /0000-0003-3247-2187/work/99485986
  • ORCID: /0000-0001-5732-6675/work/99486225