Detection of murine norovirus 1 by using plaque assay, transfection assay, and real-time reverse transcription-PCR before and after heat exposure

The correlation between the detection of murine norovirus 1 RNA by real-time reverse transcription-PCR and the infectivity by plaque assay before and after heat exposure (80 degrees C) was examined. No correlation was found in the current study. Moreover, heat inactivation had a much stronger detrimental effect on virus infectivity than on the integrity of the viral genome.

Publication year:2008

Keywords:Animals, Caliciviridae Infections, Gastroenteritis, Hot Temperature, Mice, Norovirus, RNA, Viral, Reverse Transcriptase Polymerase Chain Reaction, Temperature, Transfection, Viral Plaque Assay