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Viral capture to identify susceptibility genes (VICIS) in Brachypodium distachyon, barley and wheat

Many recent findings suggest the possibility for a physical interaction between RNA-binding proteins (RBPs), derived from the host, and vRNA of RNA-viruses during the infection, referred to as viral ribonucleoprotein complexes (RNPs) (Phillips et al. 2015). Our hypothesis is that some of these RBPs are so-called products of susceptibility genes or S genes, who aid the virus to successfully infect its host. Evidence for the importance of the RBPome in viral infection is recently provided for SINV infection in HEK293 cells (Garcia-moreno et al. 2019). Identifying such S genes and disrupting the interaction between the plant protein product of the S gene and the vRNA through knock-out or targeted mutations in the S genes could generate a plant with a more durable resistance to the virus than the use of resistance genes (van Schie & Takken 2014). We will study Brachypodium distachyon (standard line Bd21), barley (Morex) and durum wheat (Kronos) for BYDV-PAV infection. Brachypodium dystachon has a well-annotated genome and is considered a model organism for monocotyl grass species2. Both barley and wheat are interesting crops from an economic point of view.  


Date:30 Oct 2019  →  Today
Project type:PhD project