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Project
Towards diagnostic use of Neutrophil Extracellular Trap (NET) measurements in patient samples by pairing novel biomarker measurements with established microscopy readouts
Without a doubt, neutrophil extracellular traps (NETs) have led to a paradigm shift in the field of thrombosis and hemostasis, and broadly in other disease pathologies. However, in terms of measuring NETs in blood samples, the field suffers from a lack of standardization of methodology, with methods using unspecific readouts often interpreted as NETs (i.e. DNA which could come from other cell death). Our aim is thus to provide scientific backing for the use of certain assays to detect NET biomarkers as appropriate readouts for the complex biological process going on in human disease. We will do this by pairing established microscopy methods and with biochemical assays used to detect NET fragments as biomarkers. A direct comparison of assays measuring complexes of NET components will be compared to individual component measurements. This is a first step toward implementation of NETs measurements for diagnostic purposes, which is currently not possible. With a better understanding of how to accurately measure NETs in blood samples by pairing what we see by microscopy in research settings with biochemical measurements that can be applied in clinical laboratory settings, we will be able to start to bridge the gap from the bench to the bedside, where NET measurements can provide valuable insight into disease progression in a range of cardiovascular and inflammatory diseases.
Date:1 Oct 2022 → Today
Keywords:neutrophil extracellular traps, thrombosis, cardiovascular disease, standardization, diagnostics
Disciplines:Inflammation, Hematology, Diagnostics not elsewhere classified