Towards a breakthrough in applications of vibrational optical activity: an experimental and theoretical study of the crowding effects on proteins.

The goal of this research project is to collect experimental data on the effect of crowded environments on the structure of proteins by means of two innovative spectroscopic techniques, Raman optical activity (ROA) and vibrational circular dichroism (VCD). This experimental data will be compared to theoretical predictions of the effect, after which a conclusion about the nature and the importance of the influence of the crowding effect on the behavior of proteins in cellular environments can be drawn.

Keywords:VIBRATIONAL DYNAMICS, STRUCTURAL CHEMISTRY

Disciplines:Analytical chemistry, Physical chemistry