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Project

Single-objective light sheet instrument for fast and highthroughput imaging of 3D tissue

Life is 3D and so it should be studied within a matching
context. Fluorescence microscopy is the gold standard for
the imaging of 3D biological samples, but currently available
instruments are severely challenged to visualize tissues or
tissue models with a reasonable speed, suffer from high
photoxicity, or cannot operate with the multiwell plates
required for high-throughput measurements.We propose to develop a fluorescence microscope that is
capable of single-objective light-sheet fluorescence
microscopy, delivering extremely fast and sensitive 3D
imaging with a very high spatial resolution. The overall
imaging speed is over a factor 100 faster than the
workhorse confocal/multiphoton imaging, and over a factor
20 faster than spinning disk imaging. Importantly, the
system will be fully compatible with classical sample
preparations including multiwell plates.
The resulting capability will be unique within the Flemish
community and much of the international community, and
will represent a clear competitive advantage for the
investigators that apply the system to their research
questions. We anticipate a range of breakthroughs in fields
such as characterization and target validation in 3D disease
models, spatially-resolved ‘omics’, tissue and organ
development and healing, and the functional architecture
of the brain.

Date:1 May 2022 →  Today
Keywords:Fluorescence microscopy, 3D biological samples, characterization and target validation in 3D disease models, spatially-resolved ‘omics’, tissue and organ development and healing, unctional architecture of the brain
Disciplines:Image-guided interventions