Transcriptomics to study the cellular phase of Alzheimer's Disease

The early stages of Alzheimer's disease are still poorly understood. It is unknown how the different cell types react to early amyloid stress and how these changes propagate through the different brain regions. The goal of the project will be to understand the earliest steps in the progression of Alzheimer's disease, within different cell populations. Single cell transcriptomics will be applied to an Alzheimer's mouse model. Transcriptome profiling of individual cells using next-generation RNA-Seq will allow us to determine how different regions of the hippocampus react at different stages of Alzheimer’s Disease. This data will be integrated with data from other in-house and publicly-available sources which rely on technologies (such as in-situ hybridization and electrophysiology) in order to build a full spatio-temporal Alzheimer’s model.