Self-assembling Multi Layer Enzyme Network for Flow Biocatalysis

Small-scale flow reactors have increasingly gained attention in academia and industry as convenient tools for more efficient syntheses compared to the traditional batch procedures. These small-scale reactors provide uniform residence times, well-defined flow patterns, and precise reaction control. However, most of the applications to date focus on synthetic organic chemistry using traditional (precious metal based) catalysts. In this joined project between the Budapest University of Technology and Economics, the University of Ljubljana, and KU Leuven we will explore a novel microreactor for flow biocatalysis, i.e. enzyme catalysed reactions. In these microreactors the enzyme will be immobilized in the reactor in a layer-by-layer structure cross-linked by nanoparticles, resulting in a three-dimensional network. Within this network we will combine the expertise of each partner to manufacture, analyse and apply these reactors: The surface functionalization and creation of the multilayer enzyme network is performed at Budapest University of Technology and Economics. Fundamental insight into the spatial arrangement of the enzymes and nanoparticles in the layers is provided via a modelling approach by the University of Ljubljana. Finally, KU Leuven will perform the reactor characterization, i.e. determine their porosity (free-flow area ratio in the reactor) and mixing properties. Subsequently, these reactors will be applied to transamination reactions. In conclusion, upon finalization this project will deliver a novel microreactor tool enabling flow biocatalysis.

Keywords:biocatalysis, enzyme network, microreactors, multiscale modelling, transamination

Disciplines:Reacting systems