< Back to previous page


The neuroregenerative effect of human dental pulp stem cells is enhanced by inflammatory preconditioning with soluble factors contained in Leukocyte- and Platelet Rich Fibrin (R-10417)

Pathologies of the central nervous system (CNS), such as stroke are one of the main causes of death and disabilities, characterized by brain damage and loss of brain function. Unfortunately, spontaneous regeneration is insufficient and novel therapies including cell-based therapies are needed for treating these pathologies. Human dental pulp stem cells (hDPSCs) are a promising cell source that has shown encouraging results in animal models of CNS pathologies mediated by paracrine effects. Additionally, preconditioning or priming strategies enhanced their regenerative effect and secretion of soluble mediators. In this project, hDPSCs are exposed to the soluble factors contained in leukocyte- and platelet-rich fibrin (L-PRF), a clinically applied blood-derived biomaterial. L-PRF is known to contain inflammatory mediators that enhance stem cell-induced angiogenesis and immunomodulation but its effect on hDPSC-mediated neuroregeneration is unknown. I hypothesize that the neuroregenerative and -protective effect of the hDPSCs-secretome is enhanced by L-PRF priming. After determining the secretion of neurotrophins by L-PRF primed hDPSCs, the effect of the primed- and non-primed hDPSC-secretome on neural stem cell migration and proliferation as well as on neuroprotection and neurite outgrowth of neurons is evaluated. This project will contribute to insight in the therapeutic potential of hDPSCs in CNS pathologies and will generate valuable information for translational research.
Date:1 Jan 2020 →  31 Dec 2022
Keywords:human dental pulp stem cells, NEUROREGENERATION
Disciplines:Stem cell biology