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Project
Minimizing metabolic burden caused by heterologous expression of synthetic pathways: development of sensors & actuators, implementation of a genetic circuit with beta-carotene production in E. coli as case study
The expression of synthetic multi-gene pathways in non-native production hosts often results in metabolic burden, which is detrimental for productivity. A novel genetic circuit will be developed which specifically controls the expression of the heterologous pathway, so that metabolic burden can be avoided. This novel synthetic biology tool will be validated using beta-carotene production in E. coli as case study.
Date:1 Oct 2013 → 30 Sep 2017
Keywords:metabolic engineering, genetic circuit, fine tuning expression, synthetic biology, metabolic burden, Escherichia coli
Disciplines:Biomechanical engineering, Chemical product design and formulation, Biomaterials engineering, Environmental engineering and biotechnology, Separation and membrane technologies, Catalysis and reacting systems engineering, Biological system engineering, Animal biology, Industrial biotechnology, Transport phenomena, Other biotechnology, bio-engineering and biosystem engineering, Other (bio)chemical engineering, Process engineering, Other (bio)medical engineering, General chemical and biochemical engineering, Physical chemistry, Industrial microbiology, Synthetic biology