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Project

Microfluidics for single-cell omics. the C1 Single-Cell Auto Prep System, BioMark HD System and IFC controllers (Fluidigm) for single-cell analyses

Advances in whole-genome and whole-transcriptome amplification have permitted the sequencing of the DNA and RNA present in a single cell, offering a window into the extent and nature of genomic and transcriptomic heterogeneity which occurs in both normal development and disease. Single-cell approaches are revolutionising our capacity to understand the scale of genomic, epigenomic and transcriptomic diversity that occurs during the lifetime of an individual organism in health and disease. C1-microfluidics allows to isolate up to 96 intact individual cells from a suspension, and to process the cells for genome or transcriptome sequencing. This microfluidics device provides the advantage of automated capturing of single cells, and subsequent nanoliter reaction chambers to process the nucleic acids of multiple individual cells in parallel under highly standardized conditions at significantly reduced reagent costs. The C1 coupled to the BIOMARK and IFC systems, allows high throughput targeted analysis of single-cell gene expression, miRNA expression and single-cell genotyping using nanofluidics, producing more data at drastically less cost.

Date:19 May 2014 →  18 Nov 2018
Keywords:ifc-controllers, single-cell, microfluidics
Disciplines:Laboratory medicine, Medical systems biology, Molecular and cell biology