A Lattice Light Sheet microscope to image subcellular molecular dynamics, cellular forces, neuroimmune interactions and neuronal activity in 3D
All biological events, be it development, tissue homeostasis, brain and peripheral organ function, are tightly regulated by complex molecular or cellular networks. To understand how these molecules and cells operate and interact, it is best to study them in a situation that mimics their native and intact three dimensional (3D) environment as closely as possible. To understand the timing of these processes, it is crucial that we are able to collect data at very high speeds. With fluorescence microscopy we can label many different molecules and cells, but often the recording equipment suffers from limitations, related to the physical characteristics of light (limited spatial resolution due to diffraction), or limited recording speed (as in confocal microscopes). Eric Betzig, Nobel laureate in 2014, invented a novel approach in which he uses a lattice of light that is sent into biological structures. This lattice light sheet (LLS) microscope is a unique imaging solution with technical specifications that meet the 3D, live, spatiotemporal requirements and it is essential in a modern biomedical research institute. The LLS offers unprecedented image quality at very high speeds (several volumes per second) because cameras are used instead of pointscanners. The scientists in charge of this project are all in need of the fast 3D (and superresolution) imaging capabilities to push the limits of their research.