IOF POC Project SYNALI : Development and application of a Synthetic Nanobody Library (IOFPOC51)

Nanobodies are are a form of miniature-sized antibodies that are naturally present in the blood of animals of the camelidae family. Over the past 30 years they have been the subject of intense research at VUB and beyond, as they show beneficial properties for a variety of biotechnological, pharmaceutical and medical applications. Nanobodies are commonly generated by immunizing one or more camelid animals with a target molecule, followed by the construction of an immune nanobody library and the selection of affinity-matured nanobodies by biopannings.
In the SYNALI project we aim to generate a synthetic nanobody library that can be interrogated by biopannings to identify nanobody-binders against target molecules of interest without the requirement to use animals. The synthetic library will be tested against two multiple myeloma cancer antigens, which are being investigated by the host laboratory as targets for new therapeutic modalities. The synthetic nanobodies will be compared to nanobodies that are derived from immune libraries as vehicles for either targeted radionuclide therapy or CAR-T therapy against multiple myeloma.

Keywords:nanobodies, synthetic library, phage display, antibody-ingeneering

Disciplines:Medical imaging and therapy not elsewhere classified

Project type:Collaboration project