Generation of autologous T cells against acute myeloid leukemia by transduction of hematopoietic stem cells with T cell receptor genes and subsequent differentiation to T cells

Hematopoietic stem cells will be transduced to express a T Cell Receptor. This receptor will be optained by immunizing against WT1 tumor antigen. The T cell receptor genes will be cloned from WT1 speciific T cells and subsequently used for transduction of the stem cells. Stem cells will be differentiated in vitro to T cells and functionality will be assayed in an in vivo animal model