Functional genomics in human blood platelets.

v\:* {behavior:url(#default#VML);} o\:* {behavior:url(#default#VML);} w\:* {behavior:url(#default#VML);} .shape {behavior:url(#default#VML);} Normal 0 21 false false false NL X-NONE X-NONE MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:Standaardtabel; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin-top:0cm; mso-para-margin-right:0cm; mso-para-margin-bottom:10.0pt; mso-para-margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-fareast-language:EN-US;} In a quest to understand the complex mechanisms by which platelets interact with healthy and/or diseased blood vessels, many large scale genomic and proteomic studies are being performed. From these, lists of thousands of known and unknown genes/proteins have been generated, for many of which the specific function in different haemostatic processes is unknown. In this project we intend to generate genetically modified (with silenced/overexpressed genes of interest) human platelets by transplantation of lentiviral vector transduced human CD34+ hematopoietic stem cells in NOD/SCID mice. To study this, test systems will be set up to determine human platelet function in a murine background by FACS, flow chambers and in vivo thrombosis models with intravital fluorescence microscopy.

Keywords:functional genomics, blood platelets, stem cells, silencing

Disciplines:Cardiac and vascular medicine, Biochemistry and metabolism, Medical biochemistry and metabolism